L-methylfolate, a new option in psychiatric treatment, would it be linked to psoriasis relapse? / L-metilfolato, uma nova opção no tratamento psiquiátrico, estaria ligado à recaída de psoríase?

ABSTRACT We report a case of a 61-years-old woman in remission of psoriasis for 20 years. She presented recurrence of psoriasis in the form of plaques few days after taking L-methylfolate 15mg/day. The L-methylfolate was prescribed as an adjuvant for the treatment of depression in a patient with the methylenetetrahydrofolate reductase gene polymorphism (MTHFR).

RESUMO Paciente do sexo feminino, 61 anos, em remissão da psoríase por 20 anos. Apresentou recidiva de psoríase em forma de placas poucos dias após início de tratamento L-metilfolato na dose diária de 15mg. O L-metilfolato foi prescrito como terapêutica coadjuvante para tratamento de depressão em paciente portadora do polimorfismo do gene metilenotetrahidrofolato redutase.

Pre-treatment effect of myo-inositol and l-5, methyltetrahydrofolate in ovulation induction oocyte quality and pregnancy outcome in clomiphene resistance polycystic ovarian disease

Background: This study was designed to assess the treatment effect of myo-inositol and l-5, methyltetrahydrofolate in oocyte quality, pregnancy outcome in clomiphene citrate resistance PCOS cases.Methods: Authors conducted prospective open label, randomized, parallel group study in SIMS Hapur, U.P. Eligible patients full filling inclusion criteria were randomized into two groups having 25 patients in each group using myo-inositol 580mg and l-5, methyltetrahydrofolate 800mcg in treatment group and tab folic acid 400mcg in placebo group for 12 weeks. The follow-up visits are on weeks 4, 8 and 12.Results: 12 weeks later, 21 patients in treatment group restored one spontaneous menstrual cycle and 19 patients maintained the normal ovulatory activity in follow up cycle. Ovulation induction done in 18 patients with clomiphene citrate at the dose of 50mg during treatment out of which 10 conceive, as compared with only 9 women out of the 25 women (36 percent) in the placebo group ovulate (P>0.001) out of which 4 conceived. There was significant decrease in Sr. testosterone, DHEA and AMH level and estradiol level, while statically significant increase in Sr. SHBG and FSH level seen in treatment group(p<0.001).Conclusions: In the study, more number of studied patients get back to normal menstrual cyclicity, insulin-lowering activity and its intracellular role in oocyte maturation. Significant Dec seen in serum estradiol level at the day of HCG administration.

Optimization of fermentation process for metabolic engineered recombinant bacteria with methylenetetrahydrofolate reductase gene / 中国药学杂志

OBJECTIVE: The fermentation process for the metabolic engineered recombinant bacteria with methylenetetrahydrofolate reductase gene was optimized to improve L-5-methyltetrahydrofolate accumulation in the engineering bacteria. METHODS: Single- factor and orthogonal experiments were employed to investigate the optimum fermentation process of the bacteria. RESULTS: The optimum fermentation process of the bacteria was as following: sucrose was 3%, yeast extract was 1.0%, inorganic salts were NaCl-K2HPO4-MgSO4 = 1.26%:0.42%:0.02%, ammonium dihydrogen phosphate was 1.0%, inoculum amount was 5%, initial pH value was 7.5, induction time point was 2.5 h, inducer concentration was 1.4 mmol·L-1, incubation temperature was 37°C, induction duration was 6 h, addition degree of inducer was 2. Under the optimum fermentation process, the optimization results were as following: the bacteria amount had a 36.4% enhancement, the specific activity of methylenetetrahydrofolate reductase 46.8%, the L-5-methyltetrahydrofolate accumulation 66.1%, comparing with the original fermentation process. CONCLUSION: The research work provides experimental evidence for amplifying of the bacteria in agitating fermentor.